Evaluation of Morphological Malformities and hsp70 Gene Response on Aristolochic Acid Exposed Neocardina davidi (Red Shrimp).

In our study, we investigated morphological abnormalities and overexpression of one of the stress genes (hsp70) at different concentrations of Aristolochia extract. The plant material and animal for study was collected. The plant was mechanically grinded to prepare the infusion. The shrimp were acclimatized in a laboratory. Aristolochic acid (AA) exposure at concentrations 6000, 12 000, 18 000, 24 000 and 30 000 ppm to shrimp after 48 h, led to morphological malformations at 18 000 ppm concentration. Expression analysis revealed that the transcription of hsp70 was higher in 24 000 ppm (72 h) exposed N. davidi relating to control. The data obtained from the current study helps in better understanding of aristolochic acid induced toxicity, thus indicating the regulation of herbal products containing aristolochic acid in high concentration. [ABSTRACT FROM AUTHOR]