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Metabolic Alterations in Streptozotocin–nicotinamide-induced Diabetic Rats Treated with Muntingia calabura Extract via1 H-NMR-based Metabolomics.
- Source :
- Planta Medica; Aug2023, Vol. 89 Issue 9, p916-934, 19p
- Publication Year :
- 2023
-
Abstract
- Diabetes mellitus (DM) is a metabolic endocrine disorder caused by decreased insulin concentration or poor insulin response. Muntingia calabura (MC) has been used traditionally to reduce blood glucose levels. This study aims to support the traditional claim of MC as a functional food and blood-glucose-lowering regimen. The antidiabetic potential of MC is tested on a streptozotocin–nicotinamide (STZ-NA)-induced diabetic rat model by using the<superscript>1</superscript> H-NMR-based metabolomic approach. Serum biochemical analyses reveal that treatment with 250 mg/kg body weight (bw) standardized freeze-dried (FD) 50% ethanolic MC extract (MCE 250) shows favorable serum creatinine (37.77 ± 3.53 µM), urea (5.98 ± 0.84 mM) and glucose (7.36 ± 0.57 mM) lowering capacity, which was comparable to the standard drug, metformin. The clear separation between diabetic control (DC) and normal group in principal component analysis indicates the successful induction of diabetes in the STZ-NA-induced type 2 diabetic rat model. A total of nine biomarkers, including allantoin, glucose, methylnicotinamide, lactate, hippurate, creatine, dimethylamine, citrate and pyruvate are identified in ratsʼ urinary profile, discriminating DC and normal groups through orthogonal partial least squares-discriminant analysis. Induction of diabetes by STZ-NA is due to alteration in the tricarboxylic acid (TCA) cycle, gluconeogenesis pathway, pyruvate metabolism and nicotinate and nicotinamide metabolism. Oral treatment with MCE 250 in STZ-NA-induced diabetic rats shows improvement in the altered carbohydrate metabolism, cofactor and vitamin metabolic pathway, as well as purine and homocysteine metabolism. [ABSTRACT FROM AUTHOR]
- Subjects :
- BIOMARKERS
CARBOHYDRATE metabolism
METABOLOMICS
ANIMAL experimentation
GLYCEMIC control
DIABETES
HYPOGLYCEMIC agents
REGRESSION analysis
METABOLISM
RATS
DESCRIPTIVE statistics
DOSE-effect relationship in pharmacology
FACTOR analysis
RESEARCH funding
PLANT extracts
MOLECULAR structure
CREATININE
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 00320943
- Volume :
- 89
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Planta Medica
- Publication Type :
- Periodical
- Accession number :
- 169328639
- Full Text :
- https://doi.org/10.1055/a-2053-0950