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Identification of Nā-(carboxymethyl)lysine-positive cells in astroglia-rich primary cultures.
- Source :
- Journal of Neural Transmission; Oct2003, Vol. 110 Issue 10, p1077-1090, 14p
- Publication Year :
- 2003
-
Abstract
- Summary. Astroglia-rich primary cultures from rat brain were used to investigate the presence in glial cells of N<superscript>ā</superscript>-(carboxymethyl)lysine (CML), an advanced glycation endproduct. Westernblot analysis of homogenates of rat brain as well as of astroglia-rich cultures demonstrated the presence of CML-modified proteins in these samples. Immunocytochemical staining of astroglia-rich cultures revealed that only a minority of the cells in these cultures were intensively stained for CML. The staining intensity of CML-positive cells was strongly reduced, if the cells were not permeabilized, indicating that intracellular proteins were CML-modified. The CML-positive cells were identified as astrocytes and oligodendrocytes by double-labelling immunocytochemical staining for CML and the cellular markers galactocerobroside, myelin basic protein and glial fibrillary acidic protein. In contrast to other glial cells, microglial cells in astroglia-rich cultures were CML-negative. The finding that only a minority of cells in astroglia-rich cultures contains high amounts of intracellular CML-modified proteins indicates that specific properties of these CML-positive cells are responsible for the CML-formation in these cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- BRAIN
CELLS
PROTEINS
AMINO acids
NEUROGLIA
BASIC proteins
LABORATORY rats
Subjects
Details
- Language :
- English
- ISSN :
- 03009564
- Volume :
- 110
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Journal of Neural Transmission
- Publication Type :
- Academic Journal
- Accession number :
- 16708191
- Full Text :
- https://doi.org/10.1007/s00702-003-0020-z