Peculiarities of the biosynthesis ofBacillus intermediusglutamyl endopeptidase in recombinantBacillus subtiliscells during the stationary growth phase.

We studied the biosynthesis ofbacillus intermediusglutamyl endopeptidase in the recombinantbacillus subtilisstrain AJ73 ?58.21 during the stationary growth phase. We optimized the composition of the culture medium to favor effective enzyme production during the stationary growth phase and found that the nutritional requirements for glutamyl endopeptidase synthesis were different in the stationary phase and the growth retardation phase. Proteinase accumulation was activated by complex organic substrates (casein and gelatin). During the final stages of the culture growth, the enzyme production was stimulated by Ca²⁺, Mn²⁺, and Co²⁺ and inhibited by Zn²⁺, Fe²⁺, and Cu²⁺. The synthesis of glutamyl endopeptidase in the late stationary phase was not inhibited by glucose, unlike that in the trophophase during proliferation. We conclude that the regulatory mechanisms of proteinase synthesis during vegetative growth and sporulation are different. [ABSTRACT FROM AUTHOR]