Cloning and Functional Validation of Mung Bean VrPR Gene.

For the purpose of functional validation, the mung bean (Vigna radiata) VrPR gene was cloned and overexpressed in Arabidopsis thaliana. The findings revealed that the ORF of VrPR contained 1200 bp, in which 399 amino acids were encoded. Bioinformatics analysis showed that the VrPR protein belonged to the NADB Rossmann superfamily, which was one of the non-transmembrane hydrophilic proteins. VrPR was assumed to have 44 amino acid phosphorylation sites and be contained in chloroplasts. The VrPR secondary structure comprised of random coil, α helix, β angle, and extended chain, all of which were quite compatible with the anticipated tertiary structure. Moreover, analysis of the phylogenetic tree indicated that the soybean PR (Glyma.12G222200) and VrPR were closely related. Furthermore, chlorophyll content in leaves is markedly increased in Arabidopsis when VrPR is overexpressed. Our findings will serve as a reference for more functional studies on the PR genes in mung bean. [ABSTRACT FROM AUTHOR]