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Functional Differences of Grapevine Circular RNA Vv-circPTCD1 in Arabidopsis and Grapevine Callus under Abiotic Stress.

Authors :
Ren, Yi
Li, Junpeng
Liu, Jingjing
Zhang, Zhen
Song, Yue
Fan, Dongying
Liu, Minying
Zhang, Lipeng
Xu, Yuanyuan
Guo, Dinghan
He, Juan
Song, Shiren
Gao, Zhen
Ma, Chao
Source :
Plants (2223-7747); Jun2023, Vol. 12 Issue 12, p2332, 15p
Publication Year :
2023

Abstract

Circular RNAs (circRNAs) serve as covalently closed single-stranded RNAs and have been proposed to influence plant development and stress resistance. Grapevine is one of the most economically valuable fruit crops cultivated worldwide and is threatened by various abiotic stresses. Herein, we reported that a circRNA (Vv-circPTCD1) processed from the second exon of the pentatricopeptide repeat family gene PTCD1 was preferentially expressed in leaves and responded to salt and drought but not heat stress in grapevine. Additionally, the second exon sequence of PTCD1 was highly conserved, but the biogenesis of Vv-circPTCD1 is species-dependent in plants. It was further found that the overexpressed Vv-circPTCD1 can slightly decrease the abundance of the cognate host gene, and the neighboring genes are barely affected in the grapevine callus. Furthermore, we also successfully overexpressed the Vv-circPTCD1 and found that the Vv-circPTCD1 deteriorated the growth during heat, salt, and drought stresses in Arabidopsis. However, the biological effects on grapevine callus were not always consistent with those of Arabidopsis. Interestingly, we found that the transgenic plants of linear counterpart sequence also conferred the same phenotypes as those of circRNA during the three stress conditions, no matter what species it is. Those results imply that although the sequences are conserved, the biogenesis and functions of Vv-circPTCD1 are species-dependent. Our results indicate that the plant circRNA function investigation should be conducted in homologous species, which supports a valuable reference for further plant circRNA studies. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22237747
Volume :
12
Issue :
12
Database :
Complementary Index
Journal :
Plants (2223-7747)
Publication Type :
Academic Journal
Accession number :
164703668
Full Text :
https://doi.org/10.3390/plants12122332