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An alkalophilic and thermostable polygalacturonase (PGase) from Pseudomonas sp. 13159349: purification, biochemical characterization and its efficacy in olive oil extraction.
- Source :
- Biologia; Jul2023, Vol. 78 Issue 7, p1813-1824, 12p
- Publication Year :
- 2023
-
Abstract
- The present study deliberates on the biochemical characterization of a highly alkalophilic and thermostable polygalacturonase (PGase) from Pseudomonas sp. 13,159,349. Further, its efficacy in olive-oil extraction was also assessed. The PGase enzyme was produced using wheat bran solid substrate fermentation (SSF) media that was statistically optimised using Placket-Burman and RSM. The extracellularly secreted PGase was purified by gel permeation chromatography with a fold purification of 6.42 and a specific activity of 192.97 U/mg. 12% SDS-PAGE and LC-MS spectra confirmed that the enzyme has a molecular weight of 44.5 kDa. PGase exhibited optimum activity at pH 9 and 45 − 50 °C and the enzyme retained > 50% stability at alkaline pH 9, 10, as well as 45 and 50 °C even after 8 h. The enzyme's kinetic parameters (Km and Vmax) with pectin substrate were found to be 0.0663 mg/ml and 209.60 mol. min-1, respectively. Metal ions Co<superscript>2+</superscript> and Mn<superscript>2+</superscript> enhanced enzyme activity while Ag<superscript>2+</superscript>, Hg<superscript>2+</superscript>, and ethylenediaminetetraacetic acid (EDTA) reduced it. Activity could be restored by removing EDTA by dialysis, demonstrating PGase as a metalloenzyme. In addition, the use of non-ionic surfactants displayed considerable stability, while Triton-X 100 increased the activity significantly, indicating a greater interaction between enzyme and substrate. Furthermore, treatment of olive paste with 300 U and 600 U of PGase remarkedly (P < 0.05) increased the olive oil output by 1.96 to 2.35-fold (p < 0.01) in comparison to the control. It also improved the total polyphenol content by 23 and 33% (p < 0.05) respectively. Overall, the biochemical characterization of PGase has provided substantial insights into the enzyme characteristics and revealed its prospective applicability in the oil sector. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00063088
- Volume :
- 78
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Biologia
- Publication Type :
- Academic Journal
- Accession number :
- 164551578
- Full Text :
- https://doi.org/10.1007/s11756-022-01310-5