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Barcoding Populations of Pseudomonas fluorescens SBW25.
- Source :
- Journal of Molecular Evolution; Jun2023, Vol. 91 Issue 3, p254-262, 9p
- Publication Year :
- 2023
-
Abstract
- In recent years, evolutionary biologists have developed an increasing interest in the use of barcoding strategies to study eco-evolutionary dynamics of lineages within evolving populations and communities. Although barcoded populations can deliver unprecedented insight into evolutionary change, barcoding microbes presents specific technical challenges. Here, strategies are described for barcoding populations of the model bacterium Pseudomonas fluorescens SBW25, including the design and cloning of barcoded regions, preparation of libraries for amplicon sequencing, and quantification of resulting barcoded lineages. In so doing, we hope to aid the design and implementation of barcoding methodologies in a broad range of model and non-model organisms. [ABSTRACT FROM AUTHOR]
- Subjects :
- PSEUDOMONAS fluorescens
COMMUNITIES
MOLECULAR cloning
BIOLOGISTS
Subjects
Details
- Language :
- English
- ISSN :
- 00222844
- Volume :
- 91
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Journal of Molecular Evolution
- Publication Type :
- Academic Journal
- Accession number :
- 164373900
- Full Text :
- https://doi.org/10.1007/s00239-023-10103-6