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Validation and marker-assisted selection of DArT-genomic regions associated with wheat yield-related traits under normal and drought conditions.

Authors :
Hashem, Mostafa
Sandhu, Karansher Singh
Ismail, Saleh M.
Börner, Andreas
Sallam, Ahmed
Source :
Frontiers in Genetics; 2023, p1-11, 11p
Publication Year :
2023

Abstract

Quantitative trait loci (QTL) is one of the most important steps in marker-assisted selection. Few studies have validated quantitative trait loci for marker-assisted selection of yield traits under drought stress conditions in wheat. A set of 138 highly diverse wheat genotypes were tested under normal and drought stress conditions for 2 years. Plant height, heading date, spike length, grain number per spike, grain yield per spike, and 1000-kernel weight were scored. High genetic variation was found among genotypes in all traits scored under both conditions in the 2 years. The same panel was genotyped using a diversityarray technology (DArT) marker, and a genome-wide association study was performed to find alleles associated with yield traits under all conditions. A set of 191 significant DArT markerswere identified in this study. The results of the genomewide association study revealed eight common markers in wheat that were significantly associated with the same traits under both conditions in the 2 years. Out of the eight markers, seven were located on the D genome except one marker. Four validatedmarkerswere located on the 3D chromosome and found in complete linkage disequilibrium. Moreover, these four markers were significantly associated with the heading date under both conditions and the grain yield per spike under drought stress condition in the 2 years. This high-linkage disequilibrium genomic region was located within the TraesCS3D02G002400 gene model. Furthermore, of the eight validated markers, seven were previously reported to be associated with yield traits under normal and drought conditions. The results of this study provided very promising DArT markers that can be used for marker-assisted selection to genetically improve yield traits under normal and drought conditions. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16648021
Database :
Complementary Index
Journal :
Frontiers in Genetics
Publication Type :
Academic Journal
Accession number :
164206148
Full Text :
https://doi.org/10.3389/fgene.2023.1195566