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Single-cell intracellular pH dynamics regulate the cell cycle by timing the G1 exit and G2 transition.
- Source :
- Journal of Cell Science; May2023, Vol. 136 Issue 10, p1-15, 15p
- Publication Year :
- 2023
-
Abstract
- Transient changes in intracellular pH (pHi) regulate normal cell behaviors, but roles for spatiotemporal pHi dynamics in single-cell behaviors remain unclear. Here, we mapped single-cell spatiotemporal pHi dynamics during mammalian cell cycle progression both with and without cell cycle synchronization. We found that single-cell pHi is dynamic throughout the cell cycle: pHi decreases at G1/S, increases in mid-S, decreases at late S, increases at G2/M and rapidly decreases during mitosis. Importantly, although pHi is highly dynamic in dividing cells, non-dividing cells have attenuated pHi dynamics. Using two independent pHi manipulation methods, we found that low pHi inhibits completion of S phase whereas high pHi promotes both S/G2 and G2/M transitions. Our data also suggest that low pHi cues G1 exit, with decreased pHi shortening G1 and increased pHi elongating G1. Furthermore, dynamic pHi is required for S phase timing, as high pHi elongates S phase and low pHi inhibits S/G2 transition. This work reveals that spatiotemporal pHi dynamics are necessary for cell cycle progression at multiple phase transitions in single human cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL cycle
MAMMALIAN cell cycle
PHASE transitions
Subjects
Details
- Language :
- English
- ISSN :
- 00219533
- Volume :
- 136
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Journal of Cell Science
- Publication Type :
- Academic Journal
- Accession number :
- 164093316
- Full Text :
- https://doi.org/10.1242/jcs.260458