Genetic variations of duku (Lansium domesticum Corr.) from Central Java based on RAPD markers.

Duku (Lansium domesticum Corr.) is a tropical fruit crop indigenous to Indonesia and Malaysia. Duku Kalikajar is well-known for its excellent flavor, thin seeds, and enormous fruit size. Due to these characteristics, this cultivar commands the most outstanding premium. Apart from Duku Kalikajar, there are additional duku Variations in the area surrounding the districts of Purbalingga, Banyumas, and Banjarnegara. This study used RAPD markers to determine genetic variants and genetic relationships among 11 duku variations from the Banyumas, Purbalingga, and Banjarnegara districts. DNA was isolated from three regencies using the CTAB method with minor modifications. The variable noticed was the visualization of PCR-amplified bands using RAPD markers. MEGA ver 6.06 with the UPGMA algorithm was used to determine the genetic connection. PIC values of 0.5 to 0.8 were considered helpful to be very informative for the eleven duku variations. A single Variation possessed several distinct bands, including OPA-02 at 400 bp in duku Kalikajar variation 2, OPA-18 at 750 bp in duku Kembaran, OPA-18 at 800 and 900 bp in duku Wanadadi, and OPB-07 at 750 bp in duku Sigaluh. Duku variations were classified into three clusters, two central and one basal. All groups displayed a dispersed grouping pattern, implying a significant interspecies crossing across the local duku variations sampled in the Purbalingga, Banyumas, and Banjarnegara districts. [ABSTRACT FROM AUTHOR]