Simple one-step purification of nisin Z from unclarified culture broth ofLactococcus lactissubsp.lactisA164 using expanded bed ion exchange chromatography.

A simple one-step purification method, using expanded bed, ion-exchange chromatography, for the fractionation of nisin Z produced byLactococcus lactissubsp.lactisA164 was developed. The highest dynamic binding capacity (0.92) of the adsorbent was obtained at a superficial velocity of 367 cm h^-1, resulting in approx. 2.7-fold bed expansion. The range of pH for the maximum adsorption was 3-4. The isocratic elution with 0.15 m NaCl led to approx.>90% recovery. Single-step purification of nisin Z from unclarified A164 culture broth resulted in 31-fold purification with a 90% yield. [ABSTRACT FROM AUTHOR]