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Cryptosporidium proventriculi in Captive Cockatiels (Nymphicus hollandicus).

Authors :
Panegossi, Mariele Fernanda da Cruz
Widmer, Giovanni
Nagata, Walter Bertequini
Oliveira, Bruno César Miranda
Ferrari, Elis Domingos
Gomes, Jancarlo Ferreira
Meireles, Marcelo Vasconcelos
Nakamura, Alex Akira
do Santos-Doni, Thaís Rabelo
da Silveira Neto, Luiz
Bresciani, Katia Denise Saraiva
Source :
Pathogens; May2023, Vol. 12 Issue 5, p710, 11p
Publication Year :
2023

Abstract

Cockatiels (Nymphicus hollandicus) are among the most commonly sold psittacines pets. The aim of this study was to evaluate the occurrence of Cryptosporidium spp. in domestic N. hollandicus and identify risk factors for this infection. We collected fecal samples from 100 domestic cockatiels in the city of Araçatuba, São Paulo, Brazil. Feces from birds of both genders and older than two months were collected. Owners were asked to complete a questionnaire to identify how they handle and care for their birds. Based on nested PCR targeting the 18S rRNA gene, the prevalence of Cryptosporidium spp. in the cockatiels sampled was 9.00%, 6.00% based on Malachite green staining, 5.00% based on modified Kinyoun straining, and 7.00% when the Malachite green was combined with Kinyoun. Applying multivariate logistic regression to test the association between Cryptosporidium proventriculi positivity and potential predictors showed that gastrointestinal alterations was a significant predictor (p < 0.01). Amplicons from five samples were sequenced successfully and showed 100% similarity with C. proventriculi. In summary, this study demonstrates the occurrence of C. proventriculi in captive cockatiels. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20760817
Volume :
12
Issue :
5
Database :
Complementary Index
Journal :
Pathogens
Publication Type :
Academic Journal
Accession number :
163984744
Full Text :
https://doi.org/10.3390/pathogens12050710