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Co-Harboring of Beta-Lactamases and mcr-1 Genes in Escherichia coli and Klebsiella pneumoniae from Healthy Carriers and Backyard Animals in Rural Communities in Ecuador.
- Source :
- Antibiotics (2079-6382); May2023, Vol. 12 Issue 5, p856, 15p
- Publication Year :
- 2023
-
Abstract
- Few studies have addressed drug resistance of Enterobacterales in rural communities in developing countries. This study aimed to determine the coexistence of extended-spectrum β-lactamase (ESBL) and carbapenemase genes in Escherichia coli and Klebsiella pneumoniae strains carrying the mcr-1 gene in rural communities in Ecuador from healthy humans and their backyard animals. Sixty-two strains, thirty E. coli and thirty-two K. pneumoniae strains carrying the mcr-1 gene were selected from a previous study. PCR were performed for the presence of ESBLs and carbapenemase genes. The strains were further characterized, and the genetic relationship was studied with multi-locus sequencing typing (MLST) of seven housekeeping genes. Fifty-nine of the sixty-two mcr-1 isolates (95%) harbored at least on β-lactam resistance gene. The most prevalent ESBL genes were the bla<subscript>TEM</subscript> genes (present in in 80% of the E. coli strains) and the bla<subscript>SHV</subscript> gene (present in 84% of the K. pneumoniae strains). MSLT analysis revealed 28 different sequence types (ST); 15 for E. coli and 12 for K. pneumoniae, with most ST never described in humans and animals. The coexistence of mcr-1 and β-lactams resistant genes in E. coli and K. pneumoniae strains is alarming and threatens the efficacy of last-resort antibiotics. Our findings highlight backyard animals as a reservoir of mcr-1/β-lactams resistant genes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20796382
- Volume :
- 12
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Antibiotics (2079-6382)
- Publication Type :
- Academic Journal
- Accession number :
- 163938130
- Full Text :
- https://doi.org/10.3390/antibiotics12050856