PD-1/CTLA-4 Blockade Leads to Expansion of CD8+PD-1int TILs and Results in Tumor Remission in Experimental Liver Cancer.

Transferred naïve CD8 SP + sp CD45.1 SP + sp HA-specific T cells behaved similar to endogenous CD8 SP + sp CD45.2 SP + sp T cells (Fig. (b) CD4, CD8, and FoxP3 and (d) CD8, CD44, and PD-1. e Representative staining for CD8, PD-1, and Ki67 14 and 21 days after tumor induction. f Quantitative analysis at the same time points. The fate of naive tumor-specific T cells (CD8 SP + sp CD45.1 SP + sp HA-specific T cells) adoptively transferred 10 days after tumor induction was also investigated. While effector CD8 SP + sp and CD4 SP + sp Foxp3 SP - sp T cells were found almost exclusively in the tumors of treated mice, CD4 SP + sp Foxp3 SP + sp regulatory T cells (Tregs) were present in the tumors of both mouse groups (Fig. [Extracted from the article]