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Rapid detection of Heterobasidion annosum using a loop-mediated isothermal amplification assay.
- Source :
- Frontiers in Cellular & Infection Microbiology; 2023, p1-10, 10p
- Publication Year :
- 2023
-
Abstract
- Heterobasidion annosum is one of the most aggressive pathogens of Pinus forests in Europe, causing considerable economic losses. To detect H. annosum for disease diagnosis and control, we developed a loop-mediated isothermal amplification (LAMP) reaction with a primer set designed from the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) DNA sequences of H. annosum. In our study, this LAMP assay was found to be capable of efficiently amplifying the target gene within 60 min at 63°C. In specificity tests, H. annosum was positively detected, and other species were negative. The detection limit of this assay was found to be 100 pg·mL-1, and the assay was also successfully tested for use with basidiospore suspensions and wood samples. This study provides a rapid method for diagnosing root and butt rot caused by H. annosum, which will be of use in port surveillance of logs imported from Europe. [ABSTRACT FROM AUTHOR]
- Subjects :
- GLYCERALDEHYDEPHOSPHATE dehydrogenase
ROOT rots
DETECTION limit
WOOD
DNA sequencing
Subjects
Details
- Language :
- English
- ISSN :
- 22352988
- Database :
- Complementary Index
- Journal :
- Frontiers in Cellular & Infection Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 163710294
- Full Text :
- https://doi.org/10.3389/fcimb.2023.1134921