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Lewis Epitopes on Outer Membrane Vesicles of Relevance toHelicobacter pyloriPathogenesis.

Authors :
Hynes, Sean O.
Keenan, Jacqueline I.
Ferris, John A.
Annuk, Heidi
Moran, Anthony P.
Source :
Helicobacter; Apr2005, Vol. 10 Issue 2, p146-156, 11p
Publication Year :
2005

Abstract

Helicobacter pyloriextrudes protein- and lipopolysaccharide-enriched outer membrane vesicles from its cell surface which have been postulated to act to deliver virulence factors to the host. Lewis antigen expression by lipopolysaccharide ofH. pyloricells has been implicated in a number of pathogenic roles. The aim of this study was to further characterize the expression of lipopolysaccharide on the surface of these outer membrane vesicles and, in particular, expression of Lewis antigens and their association with antibody production in the host.H. pyloristrains were examined for outer membrane vesicle production using transmission electron microscopy and Lewis antigen expression probed using immunoelectron microscopy. Sera from patients were analyzed for cross-reacting anti-Lewis antibodies and, subsequently, absorbed using outer membrane vesicle preparations to remove the cross-reacting antibodies.The formation of outer membrane vesicles byH. pyloriwas observed in both in vitro and in vivo samples. Furthermore, vesicles were produced following culture in either liquid or solid medium by all strains examined. Moreover, we observed the presence of Lewis epitopes on outer membrane vesicles using immunoelectron microscopy and immunoblotting. Circulating anti-Lewis antibodies were found in the sera of gastric cancer patients but not in the sera ofH. pylori-negative control subjects. Absorption of patient sera with outer membrane vesicles decreased the levels of anti-Lewis autoantibodies.Our results demonstrate the ability ofH. pylorito generate outer membrane vesicles bearing serologically recognizable Lewis antigens on lipopolysaccharide molecules which may contribute to the chronic immune stimulation of the host. The ability of these vesicles to absorb anti-Lewis autoantibodies indicates that they may, in part, play a role in putative autoimmune aspects ofH. pyloripathogenesis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10834389
Volume :
10
Issue :
2
Database :
Complementary Index
Journal :
Helicobacter
Publication Type :
Academic Journal
Accession number :
16370874
Full Text :
https://doi.org/10.1111/j.1523-5378.2005.00302.x