Tentative function and structure prediction of putative genes in the whole genome of solventogenic clostridium acetobutylicum ATCC824 for the better elucidation of cellular metabolism.

This study involves the prediction of the function and structure of putative genes in the genome of Clostridium acetobutylicum for the better elucidation of cellular metabolism. One bacterial strain was used in this study. About 50 hypothetical genes were retrieved from the whole-genome of C. acetobutylicum. KEGG, Pfam, UniProt, InterPro, ScanProsite, SMART, ProtParam, SignalP 4.0, TargetP 2.0, NCBI blast, HHpred, CPH modeling, PSIpred were the bioinformatic tools used in this study. Out of the 50 hypothetical genes, 3 genes were predicted. The function was analyzed for CA_C0024, CA_C0082, and CA_C00412 and they showed e value of 7.7e-16, 9.9e-81, and 7.6e-34 Conclusion: Based on the bioinformatics online tools tentative function and structure for CA_C0024, CA_C0082, and CA_C00412 were predicted. The functions were found to be transmembrane protein, transferase, and virulence-associated functions for CA_C0024, CA_C0082, and CA_C00412 respectively. The prediction for the gene CA_C0024 by HHpred and CPH modeler showed similarity to transferase from gram-positive bacteria. [ABSTRACT FROM AUTHOR]