Tentative function prediction of putative genes in the whole genome of the cellulolytic bacteria Bacillus methylotrophicus for the better elucidation of cellular metabolism.

The main objective of this study involves tentative function prediction of putative genes in the whole genome of the cellulolytic bacteria Bacillus methylotrophicus. This helps to get a clear idea about the organism's genetic makeup and thereby its metabolic pathways. for the better elucidation of cellular metabolism. Materials and methods: In this study, B. methylotrophicus strain was used. About 31 hypothetical genes were collected from the whole genome of B.methylotrophicus. The sequences were collected from the KEGG database. Some of the bioinformatics tools like, Pfam, Uniport, Interpro, SCANprosite, SMART, PROTparam, SignalP 4.0 server, TargetP 2.0 server, NCBI blast, HHpred, CPH modeling, and PSIpred were the tools used in this study. Results: Among these 31 hypothetical genes, the function and structure of 2 genes were predicted. The main function that was analyzed for RBAU_0404 and RBAU_0721 and showed e values 84 and 120 respectively. Conclusion: Based on the bioinformatics online tools, the tentative function and structure of RBAU_0404 and RBAU_0721 were predicted. For these genes, the function was similar to putative hydrolyze and RNA binding protein function for RBAU_0404 and RBAU_0721 respectively. CPH modeling was used to confirm the predicted function for the gene RBAU_0404 and it showed similarity to putative hydrolyze. [ABSTRACT FROM AUTHOR]