Attenuating effects of selenium and zinc against hexavalent chromium-induced oxidative stress, hormonal instability, and placenta damage in preimplanted rats.

As a toxic metal, hexavalent chromium (CrVI) has effects on both the reproductive and endocrine systems. This study aimed to evaluate the protective effects of selenium (Se) and zinc (Zn) against the toxicity of chromium on the placenta in pregnant Wistar albino rats. Thirty pregnant Wistar rats were divided into control and four treated groups, receiving subcutaneously (s.c) on the 3rd day of pregnancy, K₂Cr₂O₇ (10 mg/kg body weight (bw)) alone, or in association with Se (0.3 mg/kg bw), ZnCl₂ (20 mg/kg bw), or both of them simultaneously. Plasma steroid hormones, placenta histoarchitecture, oxidative stress profile, and developmental parameters were investigated. These results showed that K₂Cr₂O₇ exposure induced a significant increase in the levels of both plasma estradiol (E₂) and placenta malondialdehyde (MDA), the number of fetal resorptions, and percent of post-implantation loss. On the other hand, K₂Cr₂O₇ significantly reduced developmental parameters, maternal body and placenta weight, and plasma progesterone (P) and chorionic gonadotropin hormone (β HCG) levels. However, K₂Cr₂O₇ significantly decreased the placenta activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), reduced glutathione (GSH), and nonprotein sulfhydryl (NPSH). These changes have been reinforced by histopathological evaluation of the placenta. Se and/or ZnCl₂ supplementation provoked a significant improvement in most indices. These results suggest that the co-treatment with Se or ZnCl₂ strongly opposes the placenta cytotoxicity induced by K₂Cr₂O₇ through its antioxidant action. [ABSTRACT FROM AUTHOR]