STUDYING THE QUALITATIVE COMPOSITION OF SUBSTANCES OBTAINED FROM POPLAR BUDS BY EXTRACTION AND BAROTHERMIC METHODS.

Аmong the most acute problems of the Republic of Kazakhstan, it should be noted that the creation and development of a pharmaceutical base that meets all international standards, the development of the production of original domestic drugs, and the creation of safe and environmentally friendly technologies for their production. In this direction, plants of the genus Populus (poplar) of the Salicaceae (willow) family have an advantage due to large reserves of renewable raw materials (poplar plantations in the North Kazakhstan region have industrial reserves of medicinal raw materials) and the content of various classes of compounds with a wide range of biological activity. The aim of this work was to study the qualitative composition of substances from the buds of balsam poplar Populus balsamifera obtained by extraction and barothermal methods. Objectives: to obtain the substance from the balsam poplar buds Populus balsamifera by extraction and barothermal methods; establish the qualitative composition of the obtained substances; compare the composition of substances obtained by extraction and barothermal methods. Materials and methods. Balsam poplar buds were collected in May 2021 near the village of Zarechny, North Kazakhstan region, Republic of Kazakhstan. A method for obtaining a substance from balsam poplar buds includes using freshly harvested balsam poplar buds and extraction with solvents with an increasing polarity gradient. There were used solvents: hexane, dichloromethane, and ethyl acetate. The resulting extract was evaporated. The results and conclusions: the results of the study showed the almost complete identity of the qualitative composition of the hexane extract of substances obtained by extraction and barothermal methods. In the case of ethyl acetate fractions, the difference is the presence of chalcones in the substance obtained by the barothermal method. Extraction with methylene chloride allows the separation of flavonoids, and subsequent extraction with ethyl acetate allows the separation of gibberellins. [ABSTRACT FROM AUTHOR]