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An Insight into In Vitro Antioxidant, Antimicrobial, Cytotoxic, and Apoptosis Induction Potential of Mangiferin, a Bioactive Compound Derived from Mangifera indica.
- Source :
- Plants (2223-7747); Apr2023, Vol. 12 Issue 7, p1539, 17p
- Publication Year :
- 2023
-
Abstract
- Due to their low cost, toxicity, and health risks, medicinal plants have come to be seen as useful products and sources of biologically active compounds. Mangifera indica L., a medicinal plant with a long history, has a high bioactive metabolites content. Mangiferin (C<subscript>19</subscript>H<subscript>18</subscript>O<subscript>11</subscript>) is primary isolated from M. indica's leaves, which has many pharmacological benefits. In this investigation, ultrasonic-assisted extraction with ethanol as the extraction solvent was applied to obtain mangiferin from a local type of M. indica leaves. HPLC was performed after a dichloromethane-ethyl acetate liquid–liquid fractionation method. Further, UV–vis, FTIR, and NMR spectroscopy were utilized to elucidate the structure. Interestingly, purified mangiferin displayed promising antimicrobial efficacy against a diverse variety of fungal and bacterial pathogens with MICs of 1.95–62.5 and 1.95–31.25 µg/mL, respectively. Time–kill patterns also showed that mangiferin had both bactericidal and fungicidal action. Furthermore, it exhibited strong radical dosage-dependent scavenging activity (IC<subscript>50</subscript> = 17.6 μg/mL) compared to vitamin C (Vc, IC<subscript>50</subscript> = 11.9 μg/mL), suggesting it could be developed into a viable antioxidant agent. To our delight, the IC<subscript>50</subscript> values of mangiferin for the MCF-7 and HeLa cell lines were 41.2 and 44.7 μg/mL, respectively, from MTT cell viability testing, and it was less harmful when tested against the noncancerous cell line. Notably, it significantly induced cell apoptosis in MCF-7 cells by 62.2–83.4% using annexin V-FITC/PI labeling. Hence, our findings suggest that mangiferin can be used in the medical industry to create therapeutic interventions and medication delivery systems for society. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22237747
- Volume :
- 12
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Plants (2223-7747)
- Publication Type :
- Academic Journal
- Accession number :
- 163041824
- Full Text :
- https://doi.org/10.3390/plants12071539