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Contribution of GalU to biofilm formation, motility, antibiotic and serum resistance, and pathogenicity of Salmonella Typhimurium.
- Source :
- Frontiers in Cellular & Infection Microbiology; 3/20/2023, Vol. 13, p1-11, 11p
- Publication Year :
- 2023
-
Abstract
- Introduction: Salmonella Typhimurium is the leading cause of foodborne illnesses in China, resulting in major epidemics and economic losses in recent years. Uridine diphosphate-glucose pyrophosphorylase galU plays an important role in thebiosynthesis of the bacterial envelope. Herein, we evaluated the role of galU in S. Typhimurium infection in chicken. Methods: A galU gene mutant was successfully constructed by red homologous recombination technology, and biological characteristics were studied. Results: The galU mutant strain had a rough phenotype;was defective in biofilm formation, autoagglutination, and motility; exhibited greater sensitivity to most antibiotics, serum, and egg albumen; and had lowercapacity for adhesion to chicken embryo fibroblasts cell line (DF-1). The galU mutant showed dramatically attenuated pathogenicity in chicken embryos (100,000-fold), BALB/c mice (420-fold), and chicks (100-fold). Discussion: The results imply that galU is an important virulence factor in the pathogenicity of S. Typhimurium, and it may serve a target for the development of veterinary drugs, providing a theoretical basis for the prevention and control of S. Typhimurium. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22352988
- Volume :
- 13
- Database :
- Complementary Index
- Journal :
- Frontiers in Cellular & Infection Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 162990146
- Full Text :
- https://doi.org/10.3389/fcimb.2023.1149541