Researchers from University of California Irvine Detail Findings in Molecular Endocrinology (Er Alpha/er Beta-directed Cbs Transcription Mediates E-2 Beta-stimulated Huaec H2s Production).

Human CBS promoter contains multiple estrogen-responsive elements (EREs), including one ERE preferentially binding ER alpha (alpha ERE) and three EREs preferentially binding ER beta (beta ERE), and one full ERE (alpha/beta ERE) and one half ERE (1/2 alpha/beta ERE) binding both ER alpha and ER beta. E-2 beta stimulated ER alpha/ER beta heterodimerization by recruiting ER alpha to alpha/beta EREs and beta ERE, and ER beta to beta ERE, alpha/beta EREs, and alpha ERE. Luciferase assays using reporter genes driven by human CBS promoter with a series of 5 & PRIME;-deletions identified the alpha/beta EREs binding both ER alpha and ER beta (alpha/beta ERE and 1/2 alpha/beta ERE) to be important for baseline and E-2 beta-stimulated CBS promoter activation. [Extracted from the article]