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Baicalin Reduces Immune Cell Infiltration by Inhibiting Inflammation and Protecting Tight Junctions in Ischemic Stroke Injury.
- Source :
- American Journal of Chinese Medicine; 2023, Vol. 51 Issue 2, p355-372, 18p
- Publication Year :
- 2023
-
Abstract
- Ischemic stroke is a serious health hazard that lacks effective treatment strategies. This study aims to investigate baicalin's effect on tight junctions and immune cell infiltration after ischemic stroke injury. Rat brain microvascular endothelial cells (BMECs) were treated with OGD/R to establish an in vitro model. Caspase-3, Bax, Bcl-2, zonula occludens-1 (ZO-1), occludin, claudin-5, tumor necrosis factor (TNF)- α , interleukin (IL)-6, inducible nitric oxide synthase (iNOS), Toll-like receptor (TLR) 2, TLR4, and nuclear factor-kappa B (NF- κ B) expressions were detected using qRT-PCR and western blotting. ZO-1, TNF- α , iNOS, IL6, CD31, and ZO-1 expressions were examined using immunofluorescence. A tube formation assay was performed to measure angiogenesis. An ischemia-reperfusion model in rats was established by middle cerebral artery occlusion. The infarct volume was observed using 2,3,5-triphenyltetrazolium chloride staining. TNF- α , iNOS, and IL6 levels in the serum were tested using ELISA. Flow cytometry was performed to examine immune cell inflammatory infiltration. Baicalin had no significant effect on the proliferation of normal BMECs. Baicalin inhibited apoptosis, protected against tight junction injury, and alleviated the inflammatory response in OGD/R-induced BMECs and IR rats, with the highest dose (25 μ g/mL) exerting a superior effect. Baicalin decreased the neurological function score, infarct volume, and brain water content, relieved brain morphological changes, and inhibited immune cell infiltration in vivo. In conclusion, baicalin could reduce BMECs apoptosis, protect tight junctions, and resist immune cell infiltration, thereby alleviating ischemic stroke. Our findings potentially provide a novel treatment strategy for ischemic stroke. [ABSTRACT FROM AUTHOR]
- Subjects :
- INFLAMMATION prevention
BIOLOGICAL models
ENDOTHELIAL cells
IN vitro studies
INTERLEUKINS
FLOW cytometry
REVERSE transcriptase polymerase chain reaction
STATISTICS
FLAVONOIDS
STAINS & staining (Microscopy)
NITRIC-oxide synthases
IN vivo studies
ANALYSIS of variance
ISCHEMIC stroke
CELL membranes
ANIMAL experimentation
WESTERN immunoblotting
FLUOROIMMUNOASSAY
NEOVASCULARIZATION
ONE-way analysis of variance
CELL physiology
APOPTOSIS
RATS
CEREBRAL arteries
TUMOR necrosis factors
ENZYME-linked immunosorbent assay
CELL proliferation
RESEARCH funding
DESCRIPTIVE statistics
DATA analysis
REPERFUSION injury
Subjects
Details
- Language :
- English
- ISSN :
- 0192415X
- Volume :
- 51
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- American Journal of Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 162755778
- Full Text :
- https://doi.org/10.1142/S0192415X23500180