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Regulated control of virus replication by 4-hydroxytamoxifen-induced splicing.
- Source :
- Frontiers in Microbiology; 3/13/2023, Vol. 14, p1-11, 11p
- Publication Year :
- 2023
-
Abstract
- Designing a modified virus that can be controlled to replicate will facilitate the study of pathogenic mechanisms of virus and virus-host interactions. Here, we report a universal switch element that enables precise control of virus replication after exposure to a small molecule. Inteins mediate a traceless protein splicing-ligation process, and we generate a series of modified vesicular stomatitis virus (VSV) with intein insertion into the nucleocapsid, phosphoprotein, or large RNA-dependent RNA polymerase of VSV. Two recombinant VSV, LC599 and LY1744, were screened for intein insertion in the large RNA-dependent RNA polymerase of VSV, and their replication was regulated in a dose-dependent manner with the small molecule 4-hydroxytamoxifen, which induces intein splicing to restore the VSV replication. Furthermore, in the presence of 4-hydroxytamoxifen, the intein-modified VSV LC599 replicated efficiently in an animal model like a prototype of VSV. Thus, we present a simple and highly adaptable tool for regulating virus replication. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1664302X
- Volume :
- 14
- Database :
- Complementary Index
- Journal :
- Frontiers in Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 162744124
- Full Text :
- https://doi.org/10.3389/fmicb.2023.1112580