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Increased SIRT1 Concentration Following Four Years of Selenium and Q 10 Intervention Associated with Reduced Cardiovascular Mortality at 10-Year Follow-Up—Sub-Study of a Previous Prospective Double-Blind Placebo-Controlled Randomized Clinical Trial.

Authors :
Opstad, Trine Baur
Alexander, Jan
Aaseth, Jan
Larsson, Anders
Seljeflot, Ingebjørg
Alehagen, Urban
Source :
Antioxidants; Mar2023, Vol. 12 Issue 3, p759, 13p
Publication Year :
2023

Abstract

Background: Selenium and coenzyme Q<subscript>10</subscript> (SeQ<subscript>10</subscript>) possess antioxidant and anti-inflammatory properties, potentially mediated via Sirtuin1 (SIRT1). We aimed to investigate the influence of a SeQ<subscript>10</subscript> intervention on SIRT1 concentration, with potential interactions with microRNAs. Methods: In this sub-study of a prospective double-blind placebo-controlled clinical trial, healthy subjects (mean age 76 years) were randomized to receive an active treatment (n = 165, combined 200 µg/day of Se and 200 mg/day of Q<subscript>10</subscript>) or a placebo (n = 161). SIRT1 concentration and microRNAs were measured with ELISA and PCR, respectively. Results: After four years, SIRT1 concentration was increased in the active treatment group, with mean (SD) ng/mL of 469 (436) vs. 252 (162), p < 0.001, and decreased in the placebo group, 190 (186) vs. 269 (172), p = 0.002, and the differences between the groups were significant (p = 0.006, adjusted). Those who suffered CV death during a 10-year follow-up (n = 25 and n = 52 in the active treatment and placebo groups, respectively) had significantly lower baseline SIRT1 concentrations compared to the survivors (p < 0.001). MiR-130a-3p was significantly downregulated during the intervention and correlated inversely with SIRT1 at baseline (r = −0.466, p = 0.007). Conclusion: The increased SIRT1 concentration after the SeQ<subscript>10</subscript> intervention associated with reduced CV mortality, partly mediated via miR-1303a-3p, suggests that SIRT1 is an additional mediator of the intervention, preventing vascular ageing. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20763921
Volume :
12
Issue :
3
Database :
Complementary Index
Journal :
Antioxidants
Publication Type :
Academic Journal
Accession number :
162722984
Full Text :
https://doi.org/10.3390/antiox12030759