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Radioiodination, purification, and evaluation of antihuman tumor‐derived immunoglobulin G light chain monoclonal antibody in tumor‐bearing nude mice.
- Source :
- Journal of Labelled Compounds & Radiopharmaceuticals; Mar2023, Vol. 66 Issue 3, p108-115, 8p
- Publication Year :
- 2023
-
Abstract
- We report the synthesis and biological evaluation of 131I‐labeled antihuman tumor‐derived immunoglobulin G (IgG) light chain monoclonal antibody (4E9) ([131I]I‐4E9) as a promising probe for tumor imaging. [131I]I‐4E9 was synthesized in radiochemical yield of 89.9 ± 4.7% with radiochemical purity of more than 99%. [131I]I‐4E9 showed high stability in normal saline and human serum. In cell uptake studies, [131I]I‐4E9 exhibited favorable binding affinity and high specificity in HeLa MR cells. In biodistribution studies, [131I]I‐4E9 showed high tumor uptake, high tumor/non‐tumor ratios, and specific binding in BALB/c nu/nu mice bearing human HeLa MR xenografts. Single‐photon emission computerized tomography (SPECT) imaging of [131I]I‐4E9 in the HeLa MR xenograft model demonstrated clear visualization of tumor after 48 h and confirmed specific binding in tumor. These findings suggest that [131I]I‐4E9 possesses favorable biological characteristics and warrants further investigation as a prospective probe for imaging and treatment of cancers. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03624803
- Volume :
- 66
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Journal of Labelled Compounds & Radiopharmaceuticals
- Publication Type :
- Academic Journal
- Accession number :
- 162600792
- Full Text :
- https://doi.org/10.1002/jlcr.4017