Genetic polymorphisms of TNF‐α, IL‐6, and IL‐10 in female elderly patients with chronic temporomandibular disorder pain.

Aim: To verify the association of the TNF‐α, IL‐6, and IL‐10 polymorphisms with chronic temporomandibular disorder pain development in female elderly patients. Methods: Participants were evaluated according to Diagnostic Criteria for Temporomandibular Disorders. The genomic DNA was extracted from blood according to the Salting Out method followed by a quantification using the NanoDrop spectrophotometer. The –308G/A TNF‐α polymorphism analysis was performed by the polymerase chain reaction‐restriction fragment length polymorphism technique, the determination of –174G/C IL‐6 polymorphism was performed by polymerase chain reaction, and the evaluation of the –1082A/G IL‐10 polymorphism was carried out by polymerase chain reaction‐ allele‐specific amplification. Data were analyzed using the BioEstat 5.3 software. Results: The –308G/A TNF‐α polymorphism showed a significant difference when genotypes of cases with chronic temporomandibular disorder pain and controls were compared (p =.025). There was a lack of association regarding the –174G/C IL‐6 polymorphism (p =.286) however, a positive association between the –1082A/G IL‐10 polymorphism with chronic temporomandibular disorder was observed (p =.020). Conclusion: The analyzed data of elderly subjects support the possible involvement of the GA genotype of the –308G/A TNF‐α and the AA genotype of the –1082A/G IL‐10 polymorphisms in the pathogenesis of chronic temporomandibular disorder pain. [ABSTRACT FROM AUTHOR]