Back to Search Start Over

Development of an ectopic huLiver model for Plasmodium liver stage infection.

Authors :
Samayoa-Reyes, Gabriela
Flaherty, Siobhan M.
Wickham, Kristina S.
Viera-Morilla, Sara
Strauch, Pamela M.
Roth, Alison
Padrón, Laura
Jackson, Conner M.
Meireles, Patricia
Calvo, David
Roobsoong, Wanlapa
Kangwanrangsan, Niwat
Sattabongkot, Jetsumon
Reichard, Gregory
Lafuente-Monasterio, Maria José
Rochford, Rosemary
Source :
PLoS ONE; 3/16/2023, Vol. 17 Issue 3, p1-25, 25p
Publication Year :
2023

Abstract

Early Plasmodium falciparum and P. vivax infection requires parasite replication within host hepatocytes, referred to as liver stage (LS). However, limited understanding of infection dynamics in human LS exists due to species-specificity challenges. Reported here is a reproducible, easy-to-manipulate, and moderate-cost in vivo model to study human Plasmodium LS in mice; the ectopic huLiver model. Ectopic huLiver tumors were generated through subcutaneous injection of the HC-04 cell line and shown to be infectible by both freshly dissected sporozoites and through the bite of infected mosquitoes. Evidence for complete LS development was supported by the transition to blood-stage infection in mice engrafted with human erythrocytes. Additionally, this model was successfully evaluated for its utility in testing antimalarial therapeutics, as supported by primaquine acting as a causal prophylactic against P. falciparum. Presented here is a new platform for the study of human Plasmodium infection with the potential to aid in drug discovery. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19326203
Volume :
17
Issue :
3
Database :
Complementary Index
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
162474339
Full Text :
https://doi.org/10.1371/journal.pone.0279144