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Stereospecific recognition and rapid determination of D-amino acids in human serum based on luminescent metal-organic frameworks.
- Source :
- New Journal of Chemistry; 5/7/2022, Vol. 46 Issue 17, p8029-8036, 8p
- Publication Year :
- 2022
-
Abstract
- The current luminescent metal-organic framework (MOF) based fluorescence detection mostly focuses on achiral molecules. The stereospecific recognition and determination of MOF-based detection remain challenging. A novel luminescent terbium-based MOF (Tb-MOF) based biosensing system with excellent performance for stereoselective detection of D-amino acids (D-AAs) was introduced in this paper. D-Amino acid oxidase catalyzes stereospecific oxidative deamination of D-AAs, producing H2O2. The generated H2O2 effectively quenches the fluorescence of Tb-MOF. Under optimum conditions, the D-AAs were detected with excellent efficiency. The linear working range was 0.5-100 mM for D-Alanine (D-Ala) and 0. 5-5 mM for D-Proline (D-Pro), respectively. The limit of detection (Ksv detection) for D-Ala and D-Pro was 0.09 mM and 0.14 mM, respectively. The detection can be performed within 30 min with high selectivity and long-term stability. The fluorescent sensing platform required simple fabrication and operation and was free from complicated reaction processes and expensive reagents. The present sensing system was successfully applied in D-AA detection in human serum with good recovery, indicating its potential application in detection in biological samples. [ABSTRACT FROM AUTHOR]
- Subjects :
- METAL-organic frameworks
FLUORESCENCE quenching
DETECTION limit
ACIDS
DEAMINATION
Subjects
Details
- Language :
- English
- ISSN :
- 11440546
- Volume :
- 46
- Issue :
- 17
- Database :
- Complementary Index
- Journal :
- New Journal of Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 162459352
- Full Text :
- https://doi.org/10.1039/d1nj05570d