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Ellagic acid ameliorates aging-induced renal oxidative damage through upregulating SIRT1 and NRF2.
- Source :
- BMC Complementary Medicine & Therapies; 3/10/2023, Vol. 23 Issue 1, p1-10, 10p
- Publication Year :
- 2023
-
Abstract
- Background: Aging is associated with impaired renal function and structural alterations. Oxidative stress plays a vital role in renal senescence and damage. Sirtuin 1 (SIRT1) is thought to protect cells from oxidative stress through nuclear factor erythroid 2-related factor 2 (NRF2). Ellagic acid (EA), a natural antioxidant, has been demonstrated to have renoprotective roles in vitro and in vivo. This study investigated if SIRT1 and NRF2 mediate the protective effects of EA in aged kidneys. Methods: Male Wistar rats were divided into three groups including young (4 months), old, and old + EA (25 months). Young and old groups received EA solvent, while the old + EA group was treated with EA (30 mg/kg) by gavage for 30 days. Then, the level of renal oxidative stress, SIRT1 and NRF2 expression, kidney function parameters, and histopathological indices were measured. Results: Treatment with EA significantly increased the level of antioxidant enzymes and reduced malondialdehyde concentration (P < 0.01). Moreover, EA administration remarkably upregulated mRNA and protein levels of SIRT1 and NRF2 as well as deacetylated NRF2 protein (P < 0.05). Additionally, EA treated rats improved kidney function and histopathological scores (P < 0.05). Conclusions: These findings suggest that ellagic acid exerts protective effects on aged kidneys by activating SIRT1 and NRF2 signaling. [ABSTRACT FROM AUTHOR]
- Subjects :
- IN vitro studies
PROTEINS
ALBUMINS
GLOMERULAR filtration rate
KIDNEYS
IN vivo studies
KIDNEY function tests
UREA
STAINS & staining (Microscopy)
NUCLEAR factor E2 related factor
ANIMAL experimentation
WESTERN immunoblotting
ELECTROPHORESIS
ANTIOXIDANTS
SUPEROXIDE dismutase
RNA
BLOOD collection
MANN Whitney U Test
OXIDATIVE stress
RATS
COMPARATIVE studies
MALONDIALDEHYDE
CATALASE
BENZOPYRANS
AGING
TRANSFERASES
RESEARCH funding
HISTOLOGICAL techniques
MESSENGER RNA
URINE collection & preservation
DESCRIPTIVE statistics
POLYMERASE chain reaction
DATA analysis software
ACUTE kidney failure
CREATININE
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 23
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 162358304
- Full Text :
- https://doi.org/10.1186/s12906-023-03907-y