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Diagnosis of echinococcosis by detecting circulating cell-free DNA and miRNA.
- Source :
- Expert Review of Molecular Diagnostics; Feb2023, Vol. 23 Issue 2, p133-142, 10p
- Publication Year :
- 2023
-
Abstract
- Diagnosis of echinococcosis is difficult and usually performed based on clinical findings, imaging, and serological test. However, all of them have limitations, especially in follow-up approaches. Detection of cell-free DNA (cfDNA) and micro-RNA (miRNA) is currently a hot topic for diagnosis of echinococcosis diseases. For detecting cell-free DNA in echinococcosis patient's samples such as sera, some techniques are based on next-generation sequencing (NGS), DNA-deep sequencing, some are based on PCR-based methods, and a few works related to the detection of miRNA for the diagnosis of human echinococcosis. In the detection of cell-free DNA in echinococcosis patient' samples, NGS and DNA-deep sequencing have shown high level of sensitivity, but are not suitable for routine clinical examination as they are expensive and inaccessible in the majority of endemic areas. However, PCR-based methods have shown a sensitivity of about 20–25%. To improve the sensitivity of these tests, improving the DNA extraction method, designing appropriate primers for detecting short-length fragments of circulating DNA, using a higher volume of a serum sample, and application of more sensitive PCR methods are recommended. In the field of miRNA detection, further works are recommended. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14737159
- Volume :
- 23
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Expert Review of Molecular Diagnostics
- Publication Type :
- Academic Journal
- Accession number :
- 162321263
- Full Text :
- https://doi.org/10.1080/14737159.2023.2178903