Extraction, purification and characterization of phenazine from Pseudomonas aeruginosa isolate of wastewater sources: a panacea towards clinical pathogens.

Today with the help of advancement in the biotechnological processes, researchers are concentrating more on economical and versatile ways to recover value-added products from natural resources such as waste waters. Pseudomonas aeruginosa is known to produce a broad range of secondary metabolites during its active growth phase. In this study, P aeruginosa was densely isolated from the waste dumping zone of the highly polluted Cooum River (CR), Tamil Nadu, India. The strain was identified through biochemical analysis and molecular characterization by 16S rRNA sequencing and named as JAAKPA. Enzyme profiling of the strain revealed that it had potential lipase and protease activities. Antibacterial and antifungal activities of the strain against ten clinical pathogens were tested. A significant antibacterial activity against Gram-positive strains such as methicillin-resistant Staphylococcus aureus (MRSA), Bacillus subtilis, and moderate antifungal activity against Candida albicans, were observed. The culture supernatant of JAAKPA was subjected to thin-layer chromatography and column chromatography to extract and purify the antimicrobial compound (Phenazine). The purified compound was further subjected to gas chromatography–mass spectrometry (GC-MS), fourier transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR) studies. The results revealed phenazine to be the major secondary metabolite responsible for the antimicrobial activities. [ABSTRACT FROM AUTHOR]