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Optimization of extraction of bioactive phenolics and their antioxidant potential from callus and leaf extracts of Citrus sinensis (L.) Osbeck, C. reticulata Blanco and C. maxima (Burm.) Merr.

Authors :
Manchanda, Pooja
Kaur, Harleen
Mankoo, Ramandeep Kaur
Kaur, Arshdeep
Kaur, Jashandeep
Kaur, Simranjot
Sidhu, Gurupkar Singh
Source :
Journal of Food Measurement & Characterization; Apr2023, Vol. 17 Issue 2, p1343-1353, 11p
Publication Year :
2023

Abstract

The present study was conducted with an objective of optimizing extraction conditions for achieving maximum recovery of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant potential in terms of radical scavenging activity (RSA) from ethanolic extracts of callus cultures and leaf tissues of three citrus species i.e. sweet orange (Citrus sinensis (L.) Osbeck), mandarin (Citrus reticulata Blanco) and pummelo (Citrus maxima (Burm.) Merr.). The callus was obtained from the seeds germinated under in vitro conditions. The extracts from callus cultures and leaf tissues were prepared in ethanol solutions (25%, 50% and 75%) by ultrasound-assisted extraction at different temperatures (30, 50 and 70 °C) and extraction times (10, 20 and 30 min). The results revealed that the extraction conditions of ethanol: water ratio of 50% at 50 °C for 30 min were the best in recovering maximum amounts of TPC, TFC and RSA. Analysis of variance was performed to identify various optimization conditions. A correlation analysis was performed to effect the interaction of phenols and flavonoids on antioxidant potential of callus cultures and leaf tissues in three citrus species. It was concluded that callus and leaves of the citrus species possess great potential for their use in production of bioactive compounds. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
21934126
Volume :
17
Issue :
2
Database :
Complementary Index
Journal :
Journal of Food Measurement & Characterization
Publication Type :
Academic Journal
Accession number :
162233345
Full Text :
https://doi.org/10.1007/s11694-022-01695-6