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Hydrogen Sulfide Downregulates Oncostatin M Expression via PI3K/Akt/NF-κB Signaling Processes in Neutrophil-like Differentiated HL-60 Cells.
- Source :
- Antioxidants; Feb2023, Vol. 12 Issue 2, p417, 11p
- Publication Year :
- 2023
-
Abstract
- The cytokine oncostatin M (OSM) is regarded as a critical mediator in various inflammatory responses. While the gaseous signaling molecule hydrogen sulfide (H<subscript>2</subscript>S) plays a role in a variety of pathophysiological conditions, such as hypertension, inflammatory pain, osteoarthritis, ischemic stroke, oxidative stress, retinal degeneration, and inflammatory responses, the underlying mechanism of H<subscript>2</subscript>S action on OSM expression in neutrophils needs to be clarified. In this work, we studied how H<subscript>2</subscript>S reduces OSM expression in neutrophil-like differentiated (d)HL-60 cells. To evaluate the effects of H<subscript>2</subscript>S, sodium hydrosulfide (NaHS, a donor that produces H<subscript>2</subscript>S), ELISA, real-time PCR (qPCR), immunoblotting, and immunofluorescence staining were utilized. Although exposure to granulocyte–macrophage colony-stimulating factor (GM-CSF) resulted in upregulated levels of production and mRNA expression of OSM, these upregulated levels were reduced by pretreatment with NaHS in dHL-60 cells. Similarly, the same pretreatment lowered phosphorylated levels of phosphatidylinositol 3-kinase, Akt, and nuclear factor-kB that had been elevated by stimulation with GM-CSF. Overall, our results indicated that H<subscript>2</subscript>S could be a therapeutic agent for inflammatory disorders via suppression of OSM. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20763921
- Volume :
- 12
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Antioxidants
- Publication Type :
- Academic Journal
- Accession number :
- 162084270
- Full Text :
- https://doi.org/10.3390/antiox12020417