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An electrochemical aptasensor for detection of prostate‐specific antigen‐based on carbon quantum dots‐gold nanoparticles.

An electrochemical aptasensor for detection of prostate‐specific antigen‐based on carbon quantum dots‐gold nanoparticles.

Authors :
Pourmadadi, Mehrab
Nouralishahi, Alireza
Shalbaf, Mohammad
Shabani Shayeh, Javad
Nouralishahi, Amideddin
Source :
Biotechnology & Applied Biochemistry; Feb2023, Vol. 70 Issue 1, p175-183, 9p
Publication Year :
2023

Abstract

In this work, an electrochemical aptasensor was described for the determination of prostate‐specific antigen (PSA). Aptamer chains were decorated on the surface of a glassy carbon electrode (GCE) via carbon quantum dots/Au nanoparticles (Au/CQD). Structural analysis that was used to characterize the prepared materials shows that Au/CQD nanoparticles synthesized in a spherical shape with an average size of 70 nm. Furthermore, the combination of Au nanoparticles with CQD resulted in formation of crystalline the structure of the Au/CQD composite. To study the electrochemical performance of the prepared aptasensor, cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy were used. The results show that the aptasensor has a good selectivity to PSA over other biomaterials with the time optimized about 30 min. K4[Fe(CN)6] was used as an electrochemical probe with the limit of detection about 2 fg⋅mL–1. To avoid the hazardous nature of K4[Fe(CN)6], a label‐based aptasensor was prepared using methylene blue as an electrochemical signal producer. They provide the capability of electrochemical detection in buffer phosphate solution with high sensitivity. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
08854513
Volume :
70
Issue :
1
Database :
Complementary Index
Journal :
Biotechnology & Applied Biochemistry
Publication Type :
Academic Journal
Accession number :
161968636
Full Text :
https://doi.org/10.1002/bab.2340