Back to Search
Start Over
Jiedutongluotiaogan formula restores pancreatic function by suppressing excessive autophagy and endoplasmic reticulum stress.
- Source :
- Pharmaceutical Biology; 2022, Vol. 60 Issue 1, p1542-1555, 14p
- Publication Year :
- 2022
-
Abstract
- Context: Jiedutongluotiaogan formula (JTTF), a traditional Chinese medicine (TCM), could promote islet function. However, the potential effect of JTTF on endoplasmic reticulum stress (ERS) and autophagy have not been reported. Objective: This study explores the potential effect of JTTF on ERS and autophagy in the pancreas. Materials and methods: The Zucker diabetic fatty (ZDF) rats were randomised into five groups, control, model, JTTF (1, 3, 5 g/kg/day for 12 weeks). LPS induced pancreatic b-cells were treated with JTTF (50, 100, 200 lg/mL). LPS was used to induce pancreatic β-cell injury, with cell viability and insulin secretion evaluated using MTT, glucose-stimulated insulin secretion (GSIS) assays, and PCR. Intracellular Ca<superscript>2+</superscript> concentration was measured using flow cytometry, while ERS and autophagy levels were monitored via Western blotting and/or immunostaining. Results: Compared with the model group, body weight, FGB, HbA1c, IPGTT, FINs, and HOMA-IR in JTTF treatment groups were significantly reduced. In islets cells treated with JTTF, the pancreatic islet cells in the JTTF group were increased, lipid droplets were reduced, and there was a decrease in Ca<superscript>2+</superscript> (16.67%). After JTTF intervention, PERK, p-PERK, IRE1α, p-IRE1α, ATF6, eIF2a, GRP78, p-ULK1, LC3 and p62 expression decreased, whereas Beclin1and p-mTOR expression increased. In addition, the expression of proteins related to apoptosis in the JTTF groups were lower than those in the control group. Discussion and conclusions: JTTF may alleviate pancreatic b-cell injury by inhibiting ER stress and excessive autophagy in diabetic rats. This provides a new direction for treating diabetes and restoring pancreatic dysfunction by TCM. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13880209
- Volume :
- 60
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Pharmaceutical Biology
- Publication Type :
- Academic Journal
- Accession number :
- 161889103
- Full Text :
- https://doi.org/10.1080/13880209.2022.2107019