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The complex lipid, SPPCT-800, reduces lung damage, improves pulmonary function and decreases pro-inflammatory cytokines in the murine LPS-induced acute respiratory distress syndrome (ARDS) model.
- Source :
- Pharmaceutical Biology; 2022, Vol. 60 Issue 1, p1255-1263, 9p
- Publication Year :
- 2022
-
Abstract
- Context: Acute respiratory distress syndrome (ARDS) is a highly fatal, inflammatory condition of lungs with multiple causes. There is no adequate treatment. Objective: Using the murine LPS-induced ARDS model, we investigate SPPCT-800 (a complex lipid) as treatment for ARDS. Materials and methods: C57B16/N mice received 50 lg of Escherichia coli O111:B4 lipopolysaccharide (LPS). SPPCT-800 was given as either: (1) 20 or 200 mg/kg dose 3 h after LPS; (2) 200 mg/kg (prophylactically) 30 min before LPS; or (3) eight 200 mg/kg treatments over 72 h. Controls received saline installations. Results: At 48 and 72 h, SpO<subscript>2</subscript> was 94% and 90% in controls compared to 97% and 94% in treated animals. Expiration times, at 24 and 48 h, were 160 and 137 msec for controls, but 139 and 107 msec with SPPCT-800. In BALF (24 h), cell counts were 4.7×10<superscript>6</superscript> (controls) and 2.9×10<superscript>6</superscript> (treated); protein levels were 1.5mg (controls) and 0.4mg (treated); and IL-6 was 942 ± 194 pg/mL (controls) versus 850 ± 212 pg/mL (treated) [at 72 h, 4664 ± 2591 pg/mL (controls) versus 276 ± 151 pg/mL (treated)]. Weight losses, at 48 and 72 h, were 20% and 18% (controls), but 14% and 8% (treated). Lung injury scores, at 24 and 72 h, were 1.4 and 3.0 (controls) and 0.3 and 2.2 (treated). Discussion and conclusions: SPPCT-800 was effective in reducing manifestations of ARDS. SPPCT-800 should be further investigated as therapy for ARDS, especially in longer duration or higher cumulative dose studies. [ABSTRACT FROM AUTHOR]
- Subjects :
- ADULT respiratory distress syndrome
NEUROENDOCRINE cells
LUNGS
Subjects
Details
- Language :
- English
- ISSN :
- 13880209
- Volume :
- 60
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Pharmaceutical Biology
- Publication Type :
- Academic Journal
- Accession number :
- 161889078
- Full Text :
- https://doi.org/10.1080/13880209.2022.2087689