Reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure.

Carbonate apatite (CO₃Ap) granules are useful as a bone substitute because they can be remodeled to new natural bone in a manner that conforms to the bone remodeling process. However, reconstructing large bone defects using CO₃Ap granules is difficult because of their granular shape. Therefore, we fabricated CO₃Ap honeycomb blocks (HCBs) with continuous unidirectional pores. We aimed to elucidate the tissue response and availability of CO₃Ap HCBs in the reconstruction of rabbit mandibular bone defects after marginal mandibulectomy. The percentages of the remaining CO₃Ap area and calcified bone area (newly formed bone) were estimated from the histological images. CO₃Ap area was 49.1 ± 4.9%, 30.3 ± 3.5%, and 25.5 ± 8.8%, whereas newly formed bone area was 3.0 ± 0.6%, 24.3 ± 3.3%, and 34.7 ± 4.8% at 4, 8, and 12 weeks, respectively, after implantation. Thus, CO₃Ap HCBs were gradually resorbed and replaced by new bone. The newly formed bone penetrated most of the pores in the CO₃Ap HCBs at 12 weeks after implantation. By contrast, the granulation tissue scarcely invaded the CO₃Ap HCBs. Some osteoclasts invaded the wall of CO₃Ap HCBs, making resorption pits. Furthermore, many osteoblasts were found on the newly formed bone, indicating ongoing bone remodeling. Blood vessels were also formed inside most of the pores in the CO₃Ap HCBs. These findings suggest that CO₃Ap HCBs have good osteoconductivity and can be used for the reconstruction of large mandibular bone defects. [ABSTRACT FROM AUTHOR]