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Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil.
- Source :
- Scientific Reports; 2/9/2023, p1-6, 6p
- Publication Year :
- 2023
-
Abstract
- This study analyzes the genomic findings of the first report of Salmonella isolate carrying the bla<subscript>CTX-M-55</subscript> gene, recovered from a bacteremic patient from Brazil. A bacterial isolate positive for the bla<subscript>CTX-M-55</subscript> gene was submitted to antimicrobial susceptibility testing by disk diffusion and epsilometric test. Whole genome sequencing was performed using Illumina technology. Conjugation assay was performed; plasmid sizes determined by S1-PFGE and plasmid content were investigated by hybrid assembly after MinION long reads sequencing. Isolate 288_18 was identified as sequence type ST13, resistant to ampicillin, cefotaxime, ceftazidime, cefepime, ceftriaxone, and aztreonam. A transferable IncFII plasmid sized approximately 67 kb was found to carry the bla<subscript>TEM-1</subscript> and bla<subscript>CTX-M-55</subscript> in a module consisting of IS26-bla<subscript>TEM-1B</subscript>-WbuC-bla<subscript>CTX-M-55</subscript>-IS26. In addition, an 117 kb IncI1plasmid was also identified in the 288_18 isolate, but without additional resistance genes. To the best of our knowledge, this is the first report of bla<subscript>CTX-M-55</subscript> in Salmonella isolated from human infection in Brazil. The occurrence of bla<subscript>CTX-M-55</subscript> in the IncFII epidemic plasmid in a relevant clinical human isolate of Salmonella Agona underscores the urgent need for enhanced and effective continuous surveillance for controlling its dissemination. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- Complementary Index
- Journal :
- Scientific Reports
- Publication Type :
- Academic Journal
- Accession number :
- 161857155
- Full Text :
- https://doi.org/10.1038/s41598-023-29599-5