Chryseobacterium paludis sp. nov. and Chryseobacterium foetidum sp. nov. Isolated from the Aquatic Environment, South Korea.

Two novel bacterial species CJ51^T and CJ63^T belonging to the genus Chryseobacterium were isolated from the Upo wetland and the Han River, South Korea, respectively. Cells of these strains were Gram-stain-negative, aerobic, non-motile, rod-shaped, and catalase- and oxidase-positive. Both strains were shown to grow optimally at 30 °C and pH 7 in the absence of NaCl on tryptic soy agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CJ51^T and CJ63^T belonged to the genus Chryseobacterium and were most closely related to Chryseobacterium piperi CTM^T and Chryseobacterium piscicola VQ-6316s^T with 98.47% and 98.46% 16S rRNA sequence similarities, respectively. The average nucleotide identity values of strains CJ51^T and CJ63^T with its closely related type strains Chryseobacterium piperi CTM^T and Chryseobacterium piscicola VQ-6316s^T were 81.9% and 82.1%, respectively. The major fatty acids of strains CJ51^T and CJ63^T were iso-C_15:0, iso-C_17:0 3-OH and summed feature 9 (C_16:0 10-methyl and/or iso-C_17:1ω9c). Menaquinone 6 (MK-6) was identified as the primary respiratory quinone in both strains. The major polar lipids of strains CJ51^T and CJ63^T were phosphatidylethanolamine and several unidentified amino lipids and lipids. Based on polyphasic taxonomy data, strains CJ51^T and CJ63^T represent novel species of the genus Chryseobacterium, for which names Chryseobacterium paludis sp. nov. and Chryseobacterium foetidum sp. nov. are proposed respectively. The type strains are CJ51^T (= KACC 22749^T = JCM 35632^T) and CJ63^T (= KACC 22750^T = JCM 35633^T). [ABSTRACT FROM AUTHOR]