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Network Pharmacology-Based Identification of Key Targets of Ziyin Mingmu Pills Acting on Age-Related Macular Degeneration.
- Source :
- Evidence-based Complementary & Alternative Medicine (eCAM); 2/2/2023, p1-14, 14p, 1 Black and White Photograph, 3 Diagrams, 8 Graphs
- Publication Year :
- 2023
-
Abstract
- Objective. This study is designed to find out the molecular targets of effective Chinese medicine Ziyin Mingmu pills (ZMPs) in treating age-related macular degeneration (AMD) based on network pharmacology and experimental data. Methods. A comprehensive network pharmacology strategy that consists of three sequential modules (drug-disease target molecular docking, enrichment analysis, and external verification) was carried out to identify potential targets of ZMPs acting on AMD. Results. The active ingredients of ZMPs targeting 66 genes have effects on the process of AMD. GO and KEGG pathway enrichment analyses suggested that response to oxidative stress, regulation of angiogenesis, and lipid and atherosclerosis might serve as the most important signaling pathways in ZMPs for AMD treatment. Combined with the GSE29801 dataset for further analysis, two key genes, EGFR and VEGFA, were identified. Immune infiltration analysis showed that there was a strong association between EGFR and immune cell content. In addition, images were acquired following 24 h in the scratch experiment showed that ZMPs can reduce the percentage of wound healing distance. The Western blot assay found that ZMPs increased the expression of EGFR and decreased the expression of VEGFA. Conclusion. This study sheds light on some mechanisms of ZMP therapy for AMD, particularly the effect of ZMP on the oxidative stress in RPE and cell survival and angiogenesis in AMD. We propound ZMPs as a promising strategy to intervene in the process of AMD. [ABSTRACT FROM AUTHOR]
- Subjects :
- ATHEROSCLEROSIS prevention
WOUND healing
RETINAL degeneration
HERBAL medicine
MEDICINAL plants
CLINICAL drug trials
NEOVASCULARIZATION inhibitors
ENDOTHELIAL growth factors
WESTERN immunoblotting
CELL receptors
OXIDATIVE stress
CELLULAR signal transduction
GENE expression
CELL survival
RESEARCH funding
PATHOLOGIC neovascularization
IMMUNITY
PHARMACEUTICAL chemistry
DRUG development
CELL lines
COMPUTER-assisted molecular modeling
REACTIVE oxygen species
VASCULAR endothelial growth factors
CHINESE medicine
LIPIDS
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Complementary Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 161662758
- Full Text :
- https://doi.org/10.1155/2023/5933125