Back to Search Start Over

Strategies for tailoring pH performances of glycoside hydrolases.

Authors :
Li, Shu-Fang
Cheng, Feng
Wang, Ya-Jun
Zheng, Yu-Guo
Source :
Critical Reviews in Biotechnology; Feb2023, Vol. 43 Issue 1, p121-141, 21p
Publication Year :
2023

Abstract

Glycoside hydrolases (GHs) exhibit high activity and stability under harsh conditions, such as high temperatures and extreme pHs, given their wide use in industrial biotechnology. However, strategies for improving the acidophilic and alkalophilic adaptations of GHs are poorly summarized due to the complexity of the mechanisms of these adaptations. This review not only highlights the adaptation mechanisms of acidophilic and alkalophilic GHs under extreme pH conditions, but also summarizes the recent advances in engineering the pH performances of GHs with a focus on four strategies of protein engineering, enzyme immobilization, chemical modification, and medium engineering (additives). The examples described here summarize the methods used in modulating the pH performances of GHs and indicate that methods integrated in different protein engineering techniques or methods are efficient to generate industrial biocatalysts with the desired pH performance and other adapted enzyme properties. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
07388551
Volume :
43
Issue :
1
Database :
Complementary Index
Journal :
Critical Reviews in Biotechnology
Publication Type :
Academic Journal
Accession number :
161624088
Full Text :
https://doi.org/10.1080/07388551.2021.2004084