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Towards the Laboratory Maintenance of Haemagogus janthinomys (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever.
- Source :
- Viruses (1999-4915); Jan2023, Vol. 15 Issue 1, p45, 14p
- Publication Year :
- 2023
-
Abstract
- Haemagogus (Haemagogus) janthinomys (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F<subscript>1</subscript> generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F<subscript>3</subscript> generation Hg. janthinomys eggs for the first time. A total of 62.8% (1562/2486) F<subscript>1</subscript> generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F<subscript>2</subscript> generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F<subscript>2</subscript> mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of Hg. janthinomys past the F<subscript>3</subscript> generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle. [ABSTRACT FROM AUTHOR]
- Subjects :
- YELLOW fever
BLOOD substitutes
ARTIFICIAL feeding
CHIKUNGUNYA virus
PHYTOPLASMAS
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Volume :
- 15
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Viruses (1999-4915)
- Publication Type :
- Academic Journal
- Accession number :
- 161560497
- Full Text :
- https://doi.org/10.3390/v15010045