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Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG.

Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG.

Authors :
Ali, Aamir
Vineethakumari, Chithran
Lacasa, Cristina
Lüders, Jens
Source :
Nature Communications; 1/26/2023, p1-17, 17p
Publication Year :
2023

Abstract

Organization of microtubule arrays requires spatio-temporal regulation of the microtubule nucleator γ-tubulin ring complex (γTuRC) at microtubule organizing centers (MTOCs). MTOC-localized adapter proteins are thought to recruit and activate γTuRC, but the molecular underpinnings remain obscure. Here we show that at interphase centrosomes, rather than adapters, the microtubule polymerase ch-TOG (also named chTOG or CKAP5) ultimately controls γTuRC recruitment and activation. ch-TOG co-assembles with γTuRC to stimulate nucleation around centrioles. In the absence of ch-TOG, γTuRC fails to localize to these sites, but not the centriole lumen. However, whereas some ch-TOG is stably bound at subdistal appendages, it only transiently associates with PCM. ch-TOG's dynamic behavior requires its tubulin-binding TOG domains and a C-terminal region involved in localization. In addition, ch-TOG also promotes nucleation from the Golgi. Thus, at interphase centrosomes stimulation of nucleation and γTuRC attachment are mechanistically coupled through transient recruitment of ch-TOG, and ch-TOG's nucleation-promoting activity is not restricted to centrosomes. The molecular mechanisms underpinning the organization of microtubule arrays remain unclear. Here the authors show that in human cells, the microtubule polymerase ch-TOG promotes nucleation of microtubules at the interphase centrosome and the Golgi through a mechanism that involves transient interaction with the microtubule nucleator γTuRC. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20411723
Database :
Complementary Index
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
161516560
Full Text :
https://doi.org/10.1038/s41467-023-35955-w