Back to Search
Start Over
Excretory/Secretory Proteome of Females and Males of the Hookworm Ancylostoma ceylanicum.
- Source :
- Pathogens; Jan2023, Vol. 12 Issue 1, p95, 20p
- Publication Year :
- 2023
-
Abstract
- The dynamic host-parasite mechanisms underlying hookworm infection establishment and maintenance in mammalian hosts remain poorly understood but are primarily mediated by hookworm's excretory/secretory products (ESPs), which have a wide spectrum of biological functions. We used ultra-high performance mass spectrometry to comprehensively profile and compare female and male ESPs from the zoonotic human hookworm Ancylostoma ceylanicum, which is a natural parasite of dogs, cats, and humans. We improved the genome annotation, decreasing the number of protein-coding genes by 49% while improving completeness from 92 to 96%. Compared to the previous genome annotation, we detected 11% and 10% more spectra in female and male ESPs, respectively, using this improved version, identifying a total of 795 ESPs (70% in both sexes, with the remaining sex-specific). Using functional databases (KEGG, GO and Interpro), common and sex-specific enriched functions were identified. Comparisons with the exclusively human-infective hookworm Necator americanus identified species-specific and conserved ESPs. This is the first study identifying ESPs from female and male A. ceylanicum. The findings provide a deeper understanding of hookworm protein functions that assure long-term host survival and facilitate future engineering of transgenic hookworms and analysis of regulatory elements mediating the high-level expression of ESPs. Furthermore, the findings expand the list of potential vaccine and diagnostic targets and identify biologics that can be explored for anti-inflammatory potential. [ABSTRACT FROM AUTHOR]
- Subjects :
- HOOKWORMS
ANCYLOSTOMA
DOG parasites
MASS spectrometry
MALES
Subjects
Details
- Language :
- English
- ISSN :
- 20760817
- Volume :
- 12
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Pathogens
- Publication Type :
- Academic Journal
- Accession number :
- 161475548
- Full Text :
- https://doi.org/10.3390/pathogens12010095