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Cytochrome P450 Catalyzes Benzene Ring Formation in the Biosynthesis of Trialkyl‐Substituted Aromatic Polyketides.

Authors :
Yang, Yu Meng
Zhao, Er Juan
Wei, Wanqing
Xu, Zi Fei
Shi, Jing
Wu, Xuan
Zhang, Bo
Igarashi, Yasuhiro
Jiao, Rui Hua
Liang, Yong
Tan, Ren Xiang
Ge, Hui Ming
Source :
Angewandte Chemie; 1/26/2023, Vol. 135 Issue 5, p1-7, 7p
Publication Year :
2023

Abstract

Lorneic acid and related natural products are characterized by a trialkyl‐substituted benzene ring. The formation of the aromatic core in the middle of the polyketide chain is unusual. We characterized a cytochrome P450 enzyme that can catalyze the hallmark benzene ring formation from an acyclic polyene substrate through genetic and biochemical analysis. Using this P450 as a beacon for genome mining, we obtained 12 homologous type I polyketide synthase (PKS) gene clusters, among which two gene clusters are activated and able to produce trialkyl‐substituted aromatic polyketides. Quantum chemical calculations were performed to elucidate the plausible mechanism for P450‐catalyzed benzene ring formation. Our work expands our knowledge of the catalytic diversity of cytochrome P450. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00448249
Volume :
135
Issue :
5
Database :
Complementary Index
Journal :
Angewandte Chemie
Publication Type :
Academic Journal
Accession number :
161472164
Full Text :
https://doi.org/10.1002/ange.202214026