ISOLATION FROM STEM CELL OF BREAST CANCER BY FLOW CITOMETRY.

Cancer is a disease caused by a group of cells that multiply uncontrollably and autonomously, invading other tissues locally and distance to others. Cancer stem cells are of great interest, since they are attributed the ability to originate, maintain and expand tumors, as well as to cause metastasis and tumor reappearance. The objective is to obtain cancer stem cells from the triple negative breast cancer cell line. The 4 subpopulations of the CD44 + / cd24- immunophenotype were obtained. Conclusions: Breast cancer stem cells (BCSC) have been the subject of concentrated research as potential targets for breast cancer therapies. CD44+/CD24- cell surface markers have been established as minimal biomarkers for stem cell of breast cancer BCSC. [ABSTRACT FROM AUTHOR]