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The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway.
- Source :
- Planta: An International Journal of Plant Biology; Nov2002, Vol. 216 Issue 1, p187-192, 6p
- Publication Year :
- 2002
-
Abstract
- The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene. [ABSTRACT FROM AUTHOR]
- Subjects :
- ARABIDOPSIS thaliana
GENETICS
BIOSYNTHESIS
TRANSPOSONS
PHENOTYPES
GENE mapping
Subjects
Details
- Language :
- English
- ISSN :
- 00320935
- Volume :
- 216
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Planta: An International Journal of Plant Biology
- Publication Type :
- Academic Journal
- Accession number :
- 16126298
- Full Text :
- https://doi.org/10.1007/s00425-002-0906-2